Custom Polyclonal Antibody Service 
      A reliable immunological assay demands reliable antibodies and antibody services. ShineGene provides a one-stop solution to all our customers' antibody needs. Our custom antibody services include recombinant protein expression, peptide design and synthesis, polyclonal antibody production, express polyclonal antibody production, affinity purification, conjugation, fragmentation, and immunoassay development.

Phase 1. Peptide synthesis

•  Assistance on designing peptide with respect to protein homology, antigenicity, hydrophilicity, and synthetic suitability.Free to design antigen.

• Custom FMOC Peptide synthesis up to 30 amino acids (10 mg). 4 mg of free peptide will be delivered to the customer together with the antibody.

• A cysteine will be automatically added to the C-terminus or N-terminus of the peptide, and conjugated to KLH or BSA.

•  Peptide purity is >80%. 
  Time: 2~3 weeks
  Price: around EUR15.00 for each amino acid residue
  (reference  to our website: http://www.synthesisgene.com/peptidesynthesis.html)

Phase 2. Peptide conjugation to KLH or BSA

• Conjugation of 5 mg peptide to KLH or BSA using terminal Cysteine.

    Time: 3 days
                    Price: around EUR110.00

Phase 3. Immunization and anti-sera production in rabbit

• Conjugated peptides are used to immunize rabbits.

• Ten-week protocol to produce antibodies in 2 SPF rabbits of each peptide.

•  ELISA tests until the titer reaches 1:4,000.

• 1ml of pre-immune serum and 50~80ml of final immune bleeds.

•  Final product ELISA titer>1:8000

                    Time: 10 weeks
                    Price: EUR330.00

 Phase 4. Antibody purification (optional)

• Purification of the antibody by protein A or antigen-affinity resin.

• Antibody will be dialyzed against PBS after purification if required.

                    Time: 3 days
           Price: EUR120.00  for protein A purification
           EUR240.00 for antigen-affinity purification

Total Time: around 14~16 weeks