Custom siRNA Construction Services Cat.No.:SG00105 Gene silencing of target mRNA by RNA interference (RNAi) has dramatically enhanced the arsenal of genetic tools that can be used to study gene function. RNAi is a process used by plants and invertebrates to specifically inactivate gene function. It has been shown that double-stranded RNA (dsRNA) complementary to particular messenger RNA (mRNA) can specifically inactivate gene function through the RNAi pathway in mammalian systems as well. Small interfering RNAs (siRNAs) are generated in vivo from long precursor molecules through the action of a specific RNAase III endonuclease called Dicer. Dicer initiates the cleavage of the precursor into ~22 nucleotide (nt) double-stranded duplexes with two nt 3'-overhangs and 5' phosphate termini termed short interfering RNAs (siRNAs). The siRNA is subsequently incorporated into the RNA induced silencing complex (RISC), a protein/RNA complex. The siRNA allows specific recognition of the mRNA target and the endonuclease function of the RISC results in cleavage of mRNA and thus loss of gene expression.
€240 (Additional fees for vectors)
We will design, construct, and package siRNA-expressing vector constructs targeting your gene of interest. You only provide the GenBank (or other public database) accession number to a gene sequence and choose one of our siRNA cloning and expression vectors. We can provide the following service:
1.Select siRNA sequences based on parameters that, in our experience, are most likely to select effective siRNA.
2.Synthesize and clone template sequences for the siRNA into a vector of your choice to produce siRNA-expressing vector constructs.
3.Sequence the constructs to confirm correct cloning.
4.Package and titer each construct.Deliver the plasmid and packaged constructs to you.
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