ShinePolo®
One Step RT-PCR Kitsis
a system for performing qRT-PCR in a single step, in a
single tube.RNA
does not work a template of PCR method. However, it
becomes possible to apply the method to RNA analysis by
synthesizing cDNA from RNA using reverse tramnscriptase.
This is exactly the RT-PCR method. One Step RT-PCR is
performed with this product.At One Step RT-PCR, a
reverse transcription reaction performs using Specific
Primer (Reverse).PCR performs using synthesized cDNA as
a template, and using Specific primers (Forward,Reverse)
as PCR primers. (Random Primer and Oligo dT Primer
cannot be used for a reversetranscription reaction.) ShinePolo®
is designed to allow real time RT-PCR reaction in one
step with the use of SYBR Green I®
or a detection probe, such as TaqMan®,
Molecular beacon,etc.Ficoscript®
is an engineered Moloney murine leukemia virus (MMLV)
reverse transcriptase with reduced rNase H activity and
optimized for use in a one step real-time system. PowerQ®
Taq
DNA Polymerase is a real-time quantitative PCR enzyme
formulation consisting of recombinant full-length Taq
polymerase (Engelke et al., 1990) and a
highly efficient hot start antibody that inhibits Taq
at ambient temperatures, providing automatic hot
start PCR. The increased sensitivity and specificity
exhibited by our PowerQ®
formulation
make it the perfect choice for your real-time
quantitative PCR (qPCR) applications.
One-step RT-PCR performs RT as well as PCR in a single
buffer system. The reaction proceeds without the
addition of reagents between the RT and PCR steps. This
offers the convenience of a single-tube preparation for
RT and PCR amplification. The
operation is simple and it minimizes the risk of
contamination. Amplified products are monitored in real
time, so thers is no need to verify them through
electrophoresis after PCR. This kit is suitable for
detection of tiny amount of RNA like RNA virus.
ShinePolo® is
applicable to most real-time PCR instruments, e.g. from
Applied Biosystems, Bio-Rad Laboratories, Corbett
Research, Roche,and Stratagene.
